Histopathologic Techniques Practice Test 2025 – Comprehensive Exam Prep

The use of ester wax with heavy-duty microtomes such as sliding microtomes and sledge microtomes is based on its superior properties that facilitate the slicing of tougher tissue specimens, which are often encountered in histopathology. Ester wax has a relatively high melting point and provides excellent support for the tissue, ensuring smooth and precise cuts during sectioning.

This type of wax maintains its stability under various temperature conditions and offers better compatibility with the mechanical stresses involved in operating heavier-duty microtomes. Its ability to produce high-quality sections is particularly critical when dealing with dense or fibrous tissues that require the enhanced cutting capabilities of these specific microtome types.

In contrast, other waxes, like paraffin wax, are more commonly used with standard microtomes but may not offer the required durability or cutting precision in heavier applications. Bioloid and methyl benzoate do not possess the necessary characteristics to effectively support the demands of heavy-duty microtomes, making ester wax the preferred choice in this context.